Publications

55. D’Adamo, Jinkerson, Boyd, Brown, Baxter, Peters, Posewitz (2014) Evolutionary and biotechnological implications of robust hydrogenase activity in halophilic strains of Tetraselmis. PloS ONE 9 (1), e85812

54. Davies, Jinkerson, Posewitz (2014) Toward a photosynthetic microbial platform for terpenoid engineering. Photosynthesis research, 1-20.

53. Yang, Catalanotti, Posewitz, Alric, Grossman (2014) Insights into algal fermentation. Low-Oxygen Stress in Plants, 135-163.

52. Posewitz (2014) Engineering pathways to biofuels in photoautotrophic microorganisms. Biofuels 5 (1), 67-78.

51. Jinkerson, Radakovits, Posewitz (2013) Genomic insights from the oleaginous model alga Nannochloropsis gaditana. Bioengineered 4, 1-7.

50. Work, Bentley, Scholz, D’Adamo, Gu, Vogler, Franks, Stanish, Jinkerson, and Posewitz (2013) Biocommodities from photosynthetic microorganisms. Environmental Progress & Sustainable Energy 32, 989-1001.

49. Peters, Boyd, D’Adamo,Mulder, Therien, Posewitz (2013) Hydrogenases, Nitrogenases, Anoxia, and H2 Production in water-oxidizing phototrophs. Algae for Biofuels and Energy, 37-75.

48. Meuser, Baxter, Spear, Peters, Posewitz, Boyd (2013) Contrasting patterns of community assembly in the stratified water column of Great Salt Lake, Utah. Microbial Ecology, 1-13.

47. Catalanotti, Yang, Posewitz, Grossman (2013) Fermentation metabolism and its evolution in algae. Frontiers in Plant Science, 4.

46. Radakovits, Jinkerson, Fuerstenberg, Tae, Settlage, Boore, Posewitz (2012) Draft genome sequence and genome transformation of the oleaginous alga Nannochloropis gaditana. Nature Communications 3, 686.

45. Meuser, D’Adamo, Jinkerson, Mus, Yang, Ghirardi, Seibert, Grossman, Posewitz (2012) Genetic disruption of both Chlamydomonas reinhardtii [FeFe]-hydrogenases: insight into the role of HYDA2 in H2 production. Biochemical and Biophysical Research Communications 417, 704-709.

44. Work, D’Adamo, Radakovits, Jinkerson, Posewitz (2012) Improving photosynthesis and metabolic networks for the competitive production of phototroph-derived biofuels. Current Opinion in Biotechnology 23, 290-297.

43. Work, Beliaev, Konopka, Posewitz (2012) Microbial hydrocarbons: back to the future. Biofuels 3, 103-105.

42. Price, Chan, Yoon, Yang, Qiu, Weber, Schwacke, Gross, Blouin, Lane, Reyes-Prieto, Durnford, Neilson, Lang, Burger, Steiner, Löffelhardt, Meuser, Posewitz, Ball, Arias, Henrissat, Coutinho, Rensing, Symeonidi, Doddapaneni, Green, Rajah, Boore, Bhattacharya (2012) Cyanophora paradoxa genome elucidates origin of photosynthesis in algae and plants. Science 335, 843-847.

41. Catalanotti, Dubini, Subramanian, Yang, Magneschi, Mus, Seibert, Posewitz and Grossman (2012) Altered fermentative metabolisms in Chlamydomonas reinhardtii mutants lacking PFL1 and both PFL1 and ADH1. Plant Cell 24, 692-707.

40. Magneschi, Catalanotti, Subramanian, Dubini, Yang, Posewitz, Seibert, Perata, Grossman A.R. (2012) A mutant in ADH1 of Chlamydomonas reinhardtii elicits metabolic restructuring during anaerobiosis. Plant Physiology 158, 1293-1305.

39. Meuser, Boyd, Ananyev, Karns, Radakovits, Murthy, Ghirardi, Dismukes, Peters, Posewitz  (2011) Presence and evolutionary significance of accessory FeS clusters in Chlorella variabilis NC64A [FeFe]- hydrogenase. Planta 234, 829-843.

38. Jinkerson, Subramanian, Posewitz (2011) Improving biofuel production in phototrophic microorganisms with systems biology tools. Biofuels 2, 125-144.

37. Radakovits, Eduafo, Posewitz (2011) Genetic engineering of fatty acid chain length in Phaeodactylum tricornutum. Metabolic Engineering 13, 89-95.

36. Reinsvold, Jinkerson, Radakovits, Posewitz, Basu (2011) Expression of the Artemisia annua β-caryophyllene synthase in Synechocystis sp. strain PCC6803 results in cyanobacterial sesquiterpenoid accumulation. Journal of Plant Physiology 168, 848-852.

35. Cendron, Berto, D’Adamo, Vallese, Govoni, Posewitz, Giacometti, Costantini, Zanotti (2011) Crystal structure of HydF scaffold protein provides insights into [FeFe]-hydrogenase maturation. Journal of Biological Chemistry 286, 43944- 43950.

34. Mulder, Shepard, Meuser, Joshi, King, Posewitz, Broderick, Peters (2011) Structural insights into hydrogenase maturation. Structure 19, 1038-1052.

33. Wecker, Meuser, Posewitz  and Ghirardi (2011) Design of a new biosensor for algal H2 production based on the H2-sensing system of R. Capsulatus. International Journal of Hydrogen Energy 36, 11229-11237.

32. Grossman, Catalanotti, Yang, Dubini, Magneschi, Subramanian, Posewitz, Seibert (2011) Multiple facets of anoxic metabolism and hydrogen production in the unicellular green alga Chlamydomonas reinhardtii. New Phytologist 190, 279-288.

31. Work, Radakovits, Jinkerson, Meuser, Elliott, Vinyard, Laurens, Dismukes, Posewitz  (2010) Increased lipid accumulation in the sta7-10 isoamylase mutant and increased carbohydrate accumulation in complemented strains. Eukaryotic Cell 9, 1251-1261.

30. Radakovits, Jinkerson, Darzins, Posewitz (2010) Genetic engineering of algae for enhanced biofuel production. Eukaryotic Cell 9, 486-501.

29. Meuser, Ananyev, Wittig, Ghirardi, Seibert, Dismukes, Posewitz (2009) Phenotypic diversity of hydrogen production in Chlorophycean algae reflects distinct anaerobic metabolisms. Journal of Biotechnology 142, 21-30.

28. BeerL, Boyd, Peters, Posewitz  (2009) Engineering algae for biohydrogen and biofuels. Current Opinion in Biotechnology 20, 264-271.

27. Dubini, Mus, Seibert, Grossman, Posewitz (2009) Flexibility in anaerobic metabolism as revealed in a mutant of Chlamydomonas reinhardtii lacking hydrogenase activity. Journal of Biological Chemistry 284, 7201-7213.

26. Dismukes, Carrieri, Bennette, Ananyev, Posewitz (2008) Aquatic phototrophs: efficient alternatives to land-based crops for biofuels. Current Opinion in Biotechnology 19, 235-240.

25. McGlynn, Shepard, Winslow, Naumov, Duschene, Posewitz, Broderick, Broderick, Peters (2008) HydF as a scaffold/carrier in [FeFe]-hydrogenase H-cluster biosynthesis. FEBS Letters 582, 2183-2187.

24. Nicolet, Rubach, Posewitz, Amara, Mathevon, Fontecave, Fontecilla-Camps (2008) X-ray structure of the [FeFe]-hydrogenase maturase HydE from Thermotoga maritima. Journal of Biological Chemistry 283, 18861-18872.

23. Posewitz, Mulder, Peters (2008) New frontiers in hydrogenase structure and biosynthesis. Current Chemical Biology 2, 178-199.

22. Hu, Sommerfeld, Jarvis, Seibert, Ghirardi, Posewitz, Darzins (2008) Microalgal triacylglycerols as feedstocks for biofuel production: perspectives and advances. The Plant Journal 54, 621-639.

21. Mus, Dubini, Seibert, Posewitz, Grossman (2007) Anaerobic adaptation in Chlamydomonas reinhardtii: anoxic gene expression, hydrogenase induction and metabolic pathways. Journal of Biological Chemistry 282, 25475-25486.

20. McGlynn, Ruebush, Naumov, Nagy, Dubini, King, Broderick, Posewitz, Peters (2007) In vitro activation of [FeFe] hydrogenase: new insights into hydrogenase maturation. Journal Biological Inorganic Chemistry 12, 443-447.

19. Ghirardi, Posewitz, Dubini, Maness, Seibert (2007) Algal hydrogenases and hydrogen photoproduction. Annual Review of Plant Biology 58, 71-91.

18. Grossman, Croft, Gladyshev, Merchant, Posewitz, Prochnik, Spalding (2007) Novel metabolism in Chlamydomonas through the lens of genomics. Current Opinion in Plant Biology 10, 190-198.

17. Nagy, Meuser, Plummer, Seibert, Ghirardi, King, Ahmann and Posewitz (2007) Application of gene shuffling for the rapid generation of novel [FeFe]-hydrogenase libraries. Biotechnology Letters 29, 421-430.

16. Bock, King, Blokesch, Posewitz (2006) Maturation of hydrogenases. Advances in Microbial Physiology 51, 1-72.

15. King, Posewitz, Ghirardi, Seibert (2006) Functional studies of [FeFe] hydrogenase maturation in an Escherichia coli biosynthetic system. Journal of Bacteriology 188, 2163-2172.

14. Posewitz, King, Smolinksi, Smith, Ginley, Ghirardi, Seibert (2005) Identification of genes required for hydrogenase activity in Chlamydomonas reinhardtii. Biochemical Society Transactions 33, 102-104.

13. Ghirardi, King, Posewitz, Maness-C., Fedorov, Kim, Cohen, Schulten, Seibert (2005) Approaches to developing biological H2- photoproducing organisms and processes. Biochemical Society Transactions 33, 70-72.

12. Cohen, Kim, Posewitz, Ghirardi, Schulten, Seibert, King (2005) Molecular dynamics and experimental investigation of H2 and O2 diffusion in [Fe]- hydrogenase. Biochemical Society Transactions 33, 80-82

11. Posewitz, Smolinski, Melis, Kanakagiri, Seibert, Ghirardi (2004) Disruption of an isoamylase gene in Chlamydomonas reinhardtii attenuates hydrogen production and starch accumulation. Plant Cell 16, 2151-2163.

10. Posewitz, King, Smolinski, Zhang, Seibert, Ghirardi (2004) Discovery of two novel radical S-adenosylmethionine proteins required for the assembly of an active [Fe]-hydrogenase. Journal of Biological Chemistry 279, 25711-25720.

9. Forestier, King, Zhang, Posewitz, Schwarzer, Happe, Ghirardi, Seibert M. (2003) Expression of two [Fe]-hydrogenases in Chlamydomonas reinhardtii under anaerobic conditions. European Journal of Biochemistry 270, 2750-2758.

8. Posewitz and Tempst (1999) Selective purification of phosphorylated peptides from complex protein digests using Ga(III) immobilized metal ion affinity chromatography. Analytical Chemistry 71, 2883-2892.

7. Jensen, Posewitz, Srinivasan, Winge (1998) Mapping of the DNA binding domain of the copper-responsive transcription factor Mac1 from Saccharomyces cerevisiae and possible synergism between binding sites. Journal of Biological Chemistry 273, 23805-23811.

6. Turner, Smith, Zawrotny, Summers, Posewitz, Winge (1998) Solution structure of a unique zinc domain conserved in yeast copper-regulated transcription factors. Nature Structural Biology 5, 515-518.

5. Srinivasan, Posewitz, George, Winge (1998) Characterization of the copper chaperone cox17 of Saccharomyces cerevisiae. Biochemistry 37, 7572-7577.

4. Winge, Graden, Posewitz, Martin, Jensen, Simon (1997) Sensors that mediate copper-specific activation and repression of gene expression. Journal of Biological Inorganic Chemistry 2, 2-10.

3. Posewitz, Simon, Farrell, Winge (1996) Role of the conserved histidines in the Zn module of the copper-activated transcription factors in yeast. Journal of Biological Inorganic Chemistry 1, 560-566.

2. Graden, Posewitz, Simon, George, Pickering, Winge (1996) The presence of a Cu(I)-thiolate regulatory domain in the copper-activated transcription factor Amt1. Biochemistry 35, 14583-14589.

1. Posewitz and Wilcox (1995) Properties of the Sp1 zinc finger 3 peptide: coordination chemistry, redox reactions, and metal binding competition with metallothionein. Chemical Research in Toxicology 8, 1020-1028.

Peters, Boyd, D’Adamo, Mulder, Therien, Posewitz (2012) Hydrogenases, Nitrogenases, Anoxia, and H2 Production in water-oxidizing phototrophs. In: Algal Biofuels. Michael Borowitzka (Ed.), Elsevier, in press.

Elliott, Work, Eduafo, Radakovits, Jinkerson, Darzins, Posewitz (2011) Microalgae as a feedstock for the production of biofuels: Microalgal Biochemistry, Analytical Tools, and Targeted Bioprospecting. In: Bioprocess Sciences and Technology (M-T. Liong, editor), Nova Science Publishers, New YorkY, pp. 85-115.

Posewitz, Dubini, Meuser, Seibert, Ghirardi (2009) Hydrogenases, hydrogen production and anoxia in Chlamydomonas reinhardtii. In: The Chlamydomonas Source Book, Volume 2. David Stern and Elizabeth Harris (Eds.), Elsevier, 217-255.

Seibert, King, Posewitz, Melis, Ghirardi (2008) Photosynthetic Water-Splitting for Hydrogen Production. In: Bioenergy (J. Wall Harwood, and A. Demain, Eds.) ASM Press, Washington DC, pp. 273-291.

Tempst, Erdjument-Bromage, Posewitz, Geromanos, Freckleton, Grewal, Lacomis, Lui, Philip (1999) Protein micro-characterization by mass spectrometry: sample handling and data flow. In: Mass Spectrometry in Biology and Medicine. A.L. Burlingame (Ed.); Humana Press, Totowa; pp. 121-142.

Wilcox, Bennett, Cox, Haleblian, Hill, Kowack, Liu, Merkel, Palmer, Posewitz, Roy, Wetterhahn (1999) Interaction of metallothionein with the carcinogenic metals Ni(II), Cr(VI) and As(III). In: Metallothionein IV Klaassen (Ed.); Birkhäuser, Basel; pp. 585-594.